Camerino, September 24-27 2018<\/strong><\/p>\n\n\n\nN\u2019oublions pas le principe de l\u2019\u00cfncantore :<\/strong>
Rechercher les ingr\u00e9dients dans la Nature pour pr\u00e9server naturellement l\u2019enchantement de la beaut\u00e9.<\/strong><\/p>\n\n\n\nRESUME DES ARTICLES<\/u><\/strong><\/p>\n\n\n\nTropea Onion skin: from a waste product to new valuable matrix for bioactivity<\/u><\/strong><\/p>\n\n\n\nRita Celano1, Teresa Docimo2, Anna Lisa Piccinelli1, Luca Campone1,4, Claudio Cannistr\u00e03, Rosa Di Sanzo4, Sonia Carabetta4 Luca Rastrelli1, Mariateresa Russo5
1Department of Pharmacy, University of Salerno; 2L\u2019incanto Sarl, Catanzaro; 3 H\u00f4pital Bichat – Claude-Bernard, Paris, Francia; 4 Food Chemistry, Authentication, Safety and Sensoromic Laboratory \u2013 Universit\u00e0 Mediterranea di Reggio Calabria
Introduction<\/strong>
The red onion from Tropea is known for its sweetly taste, which is mainly due to the flavonoids and the alk(en)yl cysteine sulphoxides (ACSOs) compounds. The unique red onion envelope usually considered a waste product, represent a valuable source of biomolecules therefore accurate chemical characterization of this matrix is a fundamental prerequisite for a more sustainable and productive use in pharmaceutical, cosmetical and nutraceutical formulations.
Methods<\/strong>
The outer layers of red skin onion bulbs were collected, dried and finely powdered. Exhaustive extraction of dried materials was performed by ultrasound-assisted extraction (UAE) with aqueous ethanol (70% v\/v). A UHPLC-HRMS\/MS method was developed and then applied to analyze the onion-skin extract.
Results<\/strong>
The chemical composition of onion-skin extract was determined by UHPLC-DAD-HRMS analysis. Metabolite assignments were made comparing UV\/Vis, HRMS and MS\/MS spectra with reference standards when available, or with chemo-taxonomic data reported in the literature and databases. The main compounds detected in the extract belonging to two major classes of secondary metabolites: anthocyanins and flavonols. The main flavonols found in this onion type were quercetin and quercetin glucosides, isorhamnetin glucosides, kaempferol glucoside, and, among anthocyanins, cyaniding glucosides. This extract has shown a high antioxidant activity. The activity antioxidant was measured by DPPH, ABTS and ORAC assays.
Conclusions<\/strong>
The obtained results have shown that the onion-skins are a promising and cheap source of bioactive compounds with high antioxidant activity. Furthermore, their use as a substitute for synthetic antioxidants, in addition to increasing food security, would contribute greatly to increase their added value.<\/p>\n\n\n\n
\n\n\n\nUnravelling metabolic plasticity of Glycyrrhiza glabra leaves DOP calabrese by chemical profiling<\/u><\/strong><\/p>\n\n\n\nRita Celano1, Teresa Docimo2, Anna Lisa Piccinelli1, Claudio Cannistr\u00e03, Antonio Massarotto4, Salvatore Fuda5, Luca Rastrelli1, Mariateresa Russo5
1Department of Pharmacy, University of Salerno;
2 L\u2019incanto Sarl, Catanzaro; 3 H\u00f4pital Bichat – Claude-Bernard, Paris, Francia; 4 Naturemed srl, Cosenza; 5 Food Chemistry, Authentication, Safety and Sensoromic Laboratory \u2013 Universit\u00e0 Mediterranea di Reggio Calabria
Introduction<\/strong>
Quality, aroma and flavor of Calabrian licorice roots are worldwide recognized, although it\u2019s worth noting that aerial part of this plant , usually treated as waste product, represent a surprisingly source of bioactive metabolites. Indeed distribution of metabolites depicts a peculiar biosynthetic and developmental plasticity. In this work we report the chemical profiling of surface and inner leaf content.
Methods<\/strong>
Licorice aerial parts were harvested in pre flowering and flowering time. Leaves, panicles and flowers in flowering periods were collected dried and were ultrasound extracted with EtOH \/H2O 70% three times. Vacuum dried samples were reconstituted in MeOH\/H20 1:1 for further qualitative analysis by UHPLC system. Exudates from outer licorice leaves was performed by ETOH brief immersion for 10 sec and vacuum dried , samples were analized as above.
Results \/ Discussion \/ Conclusion<\/strong>
UHPLC-ESI-HRMS analyses were performed in negative and positive ionization mode to obtain complementary information useful for characterizing the extracts of leaves, exudates, panicles and flowers in flowering periods. Metabolite assignments were made comparing UV\/Vis, HRMS and MS\/MS spectra with reference standards when available, or with chemo-taxonomic data reported in the literature and databases. The UHPLC-DAD-HRMSn analysis has allowed to identify 46 compounds belonging to two major classes of secondary metabolites: dihydrostilbenes and flavonoids. The dihydrostilbenes have the same central scaffold with different functional groups on the two aromatic rings: hydroxyl, methoxy and prenyl units. The flavonoids are glycosilated derivatives of quercetin, kaempferol, myricetin and naringenin. All extracts showed UHPLC-UV profiles qualitatively comparable except for exudate, which showed dihydrostilbenes exclusively.
Licorice aerial parts are a very promising reservoir of bioactive molecules for plant itself and humans. From an ecological perspective prenilated flavonoids in the outer leaves part represent a more efficient plant protection against insects. These molecules along with inner leaf content of glycosilated flavonoids augment flavonoid bioactivity by increasing membrane affinity and improving the interaction with target proteins.<\/p>\n\n\n\n
\n\n\n\nChemical profiling of Glycyrrhiza glabra aerial parts, a waste product of PDO<\/u><\/strong>
\u2018\u2018Licorice of Calabria\u2019\u2019<\/u><\/strong>
Rita Celano1, Teresa Docimo2, Anna Lisa Piccinelli1, Claudio Cannistr\u00e03, Antonio Massarotto4, Salvatore Fuda5, Luca Rastrelli1, Mariateresa Russo5
1Department of Pharmacy, University of Salerno; 2 L\u2019incanto Sarl, Catanzaro; 3 H\u00f4pital Bichat Claude Bernard, Paris, Francia; 4 Naturemed srl, Cosenza; 5 Food Chemistry, Authentication, Safety andSensoromic Laboratory \u2013 Universit\u00e0 Mediterranea di Reggio Calabria
Introduction<\/strong>
Glycyrrhiza glabra aerial parts are a huge amount of discarded material produced during the cultivation and harvest of PDO \u2018\u2018Licorice of Calabria\u2019\u2019 roots. This waste product represents a potential source of functional and health-promoting ingredients. The chemical profiles of exudates, leaves, panicles and flowers were determined by UHPLC-HRMS to estimate their nutraceutical potential.
Methods<\/strong>
G. glabra aerial parts were harvested in pre-flowering and flowering time from three PDO cultivations in Calabria. Exhaustive extraction of dried materials was performed by ultrasound-assisted extraction (UAE) with aqueous ethanol (70% v\/v). Exudates were recovered by immersion of leaves in ethanol (10 sec). A UHPLC-HRMS\/MS method was developed and then applied to analyze the aerial part extracts.
Results<\/strong>
Negative and positive UHPLC-HRMS analyses, combined with HRMS\/MS spectra, chemo-taxonomic data and databases, allowed the full characterization of the metabolites of Calabrian G. glabra leaf exudate, leaf, panicle and flowers. 46 phenolic compounds were identified in aerial parts belonging to two major secondary metabolite classes: dihydrostilbenes and flavonoids. Dihydrostilbenes have the same central scaffold with different functional groups on the aromatic rings (methoxy and prenyl units). Identified flavonoids are flavanones (naringenin an pinocembrin) and their prenylated derivatives, C- and O-glycosilated flavones and flavonols. Dihydrostilbenes, pinocembrin and prenylated flavanones are the most abundant bioactive compounds of aerial parts and they represent the distinctive metabolites of leaf exudates. Instead, glycosylated flavonoids are present in inner leaves and flowers.
Conclusions<\/strong>
UHPLC-HRMS profiling prove that G. glabra aerial parts are a suitable and rich source of bioactive compounds. Their main compounds have well known antimicrobic and antinflammatory properties and it is evident that these wastes represent a potential raw material to recover health-promoting ingredients for food, cosmetic and nutraceutical sectors. Further studies are in progress to evaluate their potential applications.<\/p>\n","protected":false},"excerpt":{"rendered":"La filiale italiana dell’Incanto, l’Incanto Italia, partecipa ad un progetto di ricerca finanziata dalla Comunit\u00e0 Europea per l’identificazione dei principi attivi estratti delle piante che si sviluppano nel sud dell’Italia. La ricerca ha iniziato i suoi lavori su numerosi estratti vegetali presenti in Calabria, componenti bioattivi molto interessanti dal punto di vista cosmetico, nutrizionale ma anche ambientalista. La nostra ricercatrice cos\u00ec come i ricercatori delle Universit\u00e0 di Salerno e di Reggio Calabria hanno messo in evidenza l’attivit\u00e0 antiossidante elevata della pelle della cipolla rossa di Tropea. Questa parte della cipolla, abitualmente considerata come uno scarto, \u00e8 costituita principalmente di flavono\u00efdi : requisito fondamentale per un uso pi\u00f9 sostenibile e produttivo nelle formulazioni farmaceutiche, cosmetiche e nutraceutiche. Parimenti, se la qualit\u00e0, l’aroma ed il gusto delle radici di liquirizia Calabrese sono conosciuti universalmente, le parti aeree della liquirizia abitualmente considerate come gli scarti sono state analizzate e si rivelano essere con sorpresa una sorgente di ingredienti bioattivi per la pianta lei stessa e per noi a causa della presenza di numerosi componenti che appartengono a 2 classi maggiorenni di polifenoli. Considerando le propriet\u00e0 antimicrobiche ed antinfiammatorie dei principali componenti delle parti aeree della liquirizia, \u00e8 evidente che questi \u00a0\u00bb scarti\u00a0\u00bb rappresentano una sorgente di materia prima potenziale da promovere per la salute nei settori cosmetico , nutraceutico ed alimentare. Altri studi sono in corso per valutare le loro applicazioni. I risultati di queste prime ricerche sono stati presentati ai seguenti Congressi : Congresso Mondiale annuale della Societ\u00e0 Italo-Latino- Americana di Etnomedicina, Alimentazione e Salute (SILAE) a Milazzo (Sicilia): World Ethnomedicine Summit dal 9 al 13 Settembre 2018 E Chimali – XII \u00b0 Italian Food Chemistry Congress Camerino (ITALY) 24-27 SEPTEMBRE 2018 CHIMALI – XII Italian Food Chemistry CongressCamerino, September 24-27 2018 N\u2019oublions pas le principe de l\u2019\u00cfncantore : Rechercher les ingr\u00e9dients dans la Nature pour pr\u00e9server naturellement l\u2019enchantement de la beaut\u00e9. RESUME DES ARTICLES Tropea Onion skin: from a waste product to new valuable matrix for bioactivity Rita Celano1, Teresa Docimo2, Anna Lisa Piccinelli1, Luca Campone1,4, Claudio Cannistr\u00e03, Rosa Di Sanzo4, Sonia Carabetta4 Luca Rastrelli1, Mariateresa Russo5 1Department of Pharmacy, University of Salerno; 2L\u2019incanto Sarl, Catanzaro; 3 H\u00f4pital Bichat – Claude-Bernard, Paris, Francia; 4 Food Chemistry, Authentication, Safety and Sensoromic Laboratory \u2013 Universit\u00e0 Mediterranea di Reggio Calabria Introduction The red onion from Tropea is known for its sweetly taste, which is mainly due to the flavonoids and the alk(en)yl cysteine sulphoxides (ACSOs) compounds. The unique red onion envelope usually considered a waste product, represent a valuable source of biomolecules therefore accurate chemical characterization of this matrix is a fundamental prerequisite for a more sustainable and productive use in pharmaceutical, cosmetical and nutraceutical formulations. Methods The outer layers of red skin onion bulbs were collected, dried and finely powdered. Exhaustive extraction of dried materials was performed by ultrasound-assisted extraction (UAE) with aqueous ethanol (70% v\/v). A UHPLC-HRMS\/MS method was developed and then applied to analyze the onion-skin extract. Results The chemical composition of onion-skin extract was determined by UHPLC-DAD-HRMS analysis. Metabolite assignments were made comparing UV\/Vis, HRMS and MS\/MS spectra with reference standards when available, or with chemo-taxonomic data reported in the literature and databases. The main compounds detected in the extract belonging to two major classes of secondary metabolites: anthocyanins and flavonols. The main flavonols found in this onion type were quercetin and quercetin glucosides, isorhamnetin glucosides, kaempferol glucoside, and, among anthocyanins, cyaniding glucosides. This extract has shown a high antioxidant activity. The activity antioxidant was measured by DPPH, ABTS and ORAC assays. Conclusions The obtained results have shown that the onion-skins are a promising and cheap source of bioactive compounds with high antioxidant activity. Furthermore, their use as a substitute for synthetic antioxidants, in addition to increasing food security, would contribute greatly to increase their added value. Unravelling metabolic plasticity of Glycyrrhiza glabra leaves DOP calabrese by chemical profiling Rita Celano1, Teresa Docimo2, Anna Lisa Piccinelli1, Claudio Cannistr\u00e03, Antonio Massarotto4, Salvatore Fuda5, Luca Rastrelli1, Mariateresa Russo5 1Department of Pharmacy, University of Salerno; 2 L\u2019incanto Sarl, Catanzaro; 3 H\u00f4pital Bichat – Claude-Bernard, Paris, Francia; 4 Naturemed srl, Cosenza; 5 Food Chemistry, Authentication, Safety and Sensoromic Laboratory \u2013 Universit\u00e0 Mediterranea di Reggio Calabria Introduction Quality, aroma and flavor of Calabrian licorice roots are worldwide recognized, although it\u2019s worth noting that aerial part of this plant , usually treated as waste product, represent a surprisingly source of bioactive metabolites. Indeed distribution of metabolites depicts a peculiar biosynthetic and developmental plasticity. In this work we report the chemical profiling of surface and inner leaf content. Methods Licorice aerial parts were harvested in pre flowering and flowering time. Leaves, panicles and flowers in flowering periods were collected dried and were ultrasound extracted with EtOH \/H2O 70% three times. Vacuum dried samples were reconstituted in MeOH\/H20 1:1 for further qualitative analysis by UHPLC system. Exudates from outer licorice leaves was performed by ETOH brief immersion for 10 sec and vacuum dried , samples were analized as above. Results \/ Discussion \/ Conclusion UHPLC-ESI-HRMS analyses were performed in negative and positive ionization mode to obtain complementary information useful for characterizing the extracts of leaves, exudates, panicles and flowers in flowering periods. Metabolite assignments were made comparing UV\/Vis, HRMS and MS\/MS spectra with reference standards when available, or with chemo-taxonomic data reported in the literature and databases. The UHPLC-DAD-HRMSn analysis has allowed to identify 46 compounds belonging to two major classes of secondary metabolites: dihydrostilbenes and flavonoids. The dihydrostilbenes have the same central scaffold with different functional groups on the two aromatic rings: hydroxyl, methoxy and prenyl units. The flavonoids are glycosilated derivatives of quercetin, kaempferol, myricetin and naringenin. All extracts showed UHPLC-UV profiles qualitatively comparable except for exudate, which showed dihydrostilbenes exclusively. Licorice aerial parts are a very promising reservoir of bioactive molecules for plant itself and humans. From an ecological perspective prenilated flavonoids in the outer leaves part represent a more efficient plant protection against insects. These molecules along with inner leaf content of glycosilated flavonoids augment flavonoid bioactivity by increasing membrane affinity and improving<\/p>\n","protected":false},"author":1,"featured_media":2466,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"ocean_post_layout":"","ocean_both_sidebars_style":"","ocean_both_sidebars_content_width":0,"ocean_both_sidebars_sidebars_width":0,"ocean_sidebar":"0","ocean_second_sidebar":"0","ocean_disable_margins":"enable","ocean_add_body_class":"","ocean_shortcode_before_top_bar":"","ocean_shortcode_after_top_bar":"","ocean_shortcode_before_header":"","ocean_shortcode_after_header":"","ocean_has_shortcode":"","ocean_shortcode_after_title":"","ocean_shortcode_before_footer_widgets":"","ocean_shortcode_after_footer_widgets":"","ocean_shortcode_before_footer_bottom":"","ocean_shortcode_after_footer_bottom":"","ocean_display_top_bar":"default","ocean_display_header":"default","ocean_header_style":"","ocean_center_header_left_menu":"0","ocean_custom_header_template":"0","ocean_custom_logo":0,"ocean_custom_retina_logo":0,"ocean_custom_logo_max_width":0,"ocean_custom_logo_tablet_max_width":0,"ocean_custom_logo_mobile_max_width":0,"ocean_custom_logo_max_height":0,"ocean_custom_logo_tablet_max_height":0,"ocean_custom_logo_mobile_max_height":0,"ocean_header_custom_menu":"0","ocean_menu_typo_font_family":"0","ocean_menu_typo_font_subset":"","ocean_menu_typo_font_size":0,"ocean_menu_typo_font_size_tablet":0,"ocean_menu_typo_font_size_mobile":0,"ocean_menu_typo_font_size_unit":"px","ocean_menu_typo_font_weight":"","ocean_menu_typo_font_weight_tablet":"","ocean_menu_typo_font_weight_mobile":"","ocean_menu_typo_transform":"","ocean_menu_typo_transform_tablet":"","ocean_menu_typo_transform_mobile":"","ocean_menu_typo_line_height":0,"ocean_menu_typo_line_height_tablet":0,"ocean_menu_typo_line_height_mobile":0,"ocean_menu_typo_line_height_unit":"","ocean_menu_typo_spacing":0,"ocean_menu_typo_spacing_tablet":0,"ocean_menu_typo_spacing_mobile":0,"ocean_menu_typo_spacing_unit":"","ocean_menu_link_color":"","ocean_menu_link_color_hover":"","ocean_menu_link_color_active":"","ocean_menu_link_background":"","ocean_menu_link_hover_background":"","ocean_menu_link_active_background":"","ocean_menu_social_links_bg":"","ocean_menu_social_hover_links_bg":"","ocean_menu_social_links_color":"","ocean_menu_social_hover_links_color":"","ocean_disable_title":"default","ocean_disable_heading":"default","ocean_post_title":"","ocean_post_subheading":"","ocean_post_title_style":"","ocean_post_title_background_color":"","ocean_post_title_background":0,"ocean_post_title_bg_image_position":"","ocean_post_title_bg_image_attachment":"","ocean_post_title_bg_image_repeat":"","ocean_post_title_bg_image_size":"","ocean_post_title_height":0,"ocean_post_title_bg_overlay":0.5,"ocean_post_title_bg_overlay_color":"","ocean_disable_breadcrumbs":"default","ocean_breadcrumbs_color":"","ocean_breadcrumbs_separator_color":"","ocean_breadcrumbs_links_color":"","ocean_breadcrumbs_links_hover_color":"","ocean_display_footer_widgets":"default","ocean_display_footer_bottom":"default","ocean_custom_footer_template":"0","_glsr_average":0,"_glsr_ranking":0,"_glsr_reviews":0,"ocean_post_oembed":"","ocean_post_self_hosted_media":"","ocean_post_video_embed":"","ocean_link_format":"","ocean_link_format_target":"self","ocean_quote_format":"","ocean_quote_format_link":"post","ocean_gallery_link_images":"off","ocean_gallery_id":[],"footnotes":""},"categories":[38],"tags":[],"class_list":["post-1810","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-attualita","entry","has-media","owp-thumbs-layout-horizontal","owp-btn-normal","owp-tabs-layout-horizontal","has-no-thumbnails"],"yoast_head":"\n
La filiale italiana dell'Incanto, l'Incanto Italia, partecipa ad un progetto di ricerca finanziata dalla Comunit\u00e0 Europea - l'incantore<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.lincantore.com\/?p=1810&lang=it\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"La filiale italiana dell'Incanto, l'Incanto Italia, partecipa ad un progetto di ricerca finanziata dalla Comunit\u00e0 Europea - l'incantore\" \/>\n<meta property=\"og:description\" content=\"La filiale italiana dell’Incanto, l’Incanto Italia, partecipa ad un progetto di ricerca finanziata dalla Comunit\u00e0 Europea per l’identificazione dei principi attivi estratti delle piante che si sviluppano nel sud dell’Italia. La ricerca ha iniziato i suoi lavori su numerosi estratti vegetali presenti in Calabria, componenti bioattivi molto interessanti dal punto di vista cosmetico, nutrizionale ma anche ambientalista. La nostra ricercatrice cos\u00ec come i ricercatori delle Universit\u00e0 di Salerno e di Reggio Calabria hanno messo in evidenza l’attivit\u00e0 antiossidante elevata della pelle della cipolla rossa di Tropea. Questa parte della cipolla, abitualmente considerata come uno scarto, \u00e8 costituita principalmente di flavono\u00efdi : requisito fondamentale per un uso pi\u00f9 sostenibile e produttivo nelle formulazioni farmaceutiche, cosmetiche e nutraceutiche. Parimenti, se la qualit\u00e0, l’aroma ed il gusto delle radici di liquirizia Calabrese sono conosciuti universalmente, le parti aeree della liquirizia abitualmente considerate come gli scarti sono state analizzate e si rivelano essere con sorpresa una sorgente di ingredienti bioattivi per la pianta lei stessa e per noi a causa della presenza di numerosi componenti che appartengono a 2 classi maggiorenni di polifenoli. Considerando le propriet\u00e0 antimicrobiche ed antinfiammatorie dei principali componenti delle parti aeree della liquirizia, \u00e8 evidente che questi \u00a0\u00bb scarti\u00a0\u00bb rappresentano una sorgente di materia prima potenziale da promovere per la salute nei settori cosmetico , nutraceutico ed alimentare. Altri studi sono in corso per valutare le loro applicazioni. I risultati di queste prime ricerche sono stati presentati ai seguenti Congressi : Congresso Mondiale annuale della Societ\u00e0 Italo-Latino- Americana di Etnomedicina, Alimentazione e Salute (SILAE) a Milazzo (Sicilia): World Ethnomedicine Summit dal 9 al 13 Settembre 2018 E Chimali – XII \u00b0 Italian Food Chemistry Congress Camerino (ITALY) 24-27 SEPTEMBRE 2018 CHIMALI – XII Italian Food Chemistry CongressCamerino, September 24-27 2018 N\u2019oublions pas le principe de l\u2019\u00cfncantore : Rechercher les ingr\u00e9dients dans la Nature pour pr\u00e9server naturellement l\u2019enchantement de la beaut\u00e9. RESUME DES ARTICLES Tropea Onion skin: from a waste product to new valuable matrix for bioactivity Rita Celano1, Teresa Docimo2, Anna Lisa Piccinelli1, Luca Campone1,4, Claudio Cannistr\u00e03, Rosa Di Sanzo4, Sonia Carabetta4 Luca Rastrelli1, Mariateresa Russo5 1Department of Pharmacy, University of Salerno; 2L\u2019incanto Sarl, Catanzaro; 3 H\u00f4pital Bichat – Claude-Bernard, Paris, Francia; 4 Food Chemistry, Authentication, Safety and Sensoromic Laboratory \u2013 Universit\u00e0 Mediterranea di Reggio Calabria Introduction The red onion from Tropea is known for its sweetly taste, which is mainly due to the flavonoids and the alk(en)yl cysteine sulphoxides (ACSOs) compounds. The unique red onion envelope usually considered a waste product, represent a valuable source of biomolecules therefore accurate chemical characterization of this matrix is a fundamental prerequisite for a more sustainable and productive use in pharmaceutical, cosmetical and nutraceutical formulations. Methods The outer layers of red skin onion bulbs were collected, dried and finely powdered. Exhaustive extraction of dried materials was performed by ultrasound-assisted extraction (UAE) with aqueous ethanol (70% v\/v). A UHPLC-HRMS\/MS method was developed and then applied to analyze the onion-skin extract. Results The chemical composition of onion-skin extract was determined by UHPLC-DAD-HRMS analysis. Metabolite assignments were made comparing UV\/Vis, HRMS and MS\/MS spectra with reference standards when available, or with chemo-taxonomic data reported in the literature and databases. The main compounds detected in the extract belonging to two major classes of secondary metabolites: anthocyanins and flavonols. The main flavonols found in this onion type were quercetin and quercetin glucosides, isorhamnetin glucosides, kaempferol glucoside, and, among anthocyanins, cyaniding glucosides. This extract has shown a high antioxidant activity. The activity antioxidant was measured by DPPH, ABTS and ORAC assays. Conclusions The obtained results have shown that the onion-skins are a promising and cheap source of bioactive compounds with high antioxidant activity. Furthermore, their use as a substitute for synthetic antioxidants, in addition to increasing food security, would contribute greatly to increase their added value. Unravelling metabolic plasticity of Glycyrrhiza glabra leaves DOP calabrese by chemical profiling Rita Celano1, Teresa Docimo2, Anna Lisa Piccinelli1, Claudio Cannistr\u00e03, Antonio Massarotto4, Salvatore Fuda5, Luca Rastrelli1, Mariateresa Russo5 1Department of Pharmacy, University of Salerno; 2 L\u2019incanto Sarl, Catanzaro; 3 H\u00f4pital Bichat – Claude-Bernard, Paris, Francia; 4 Naturemed srl, Cosenza; 5 Food Chemistry, Authentication, Safety and Sensoromic Laboratory \u2013 Universit\u00e0 Mediterranea di Reggio Calabria Introduction Quality, aroma and flavor of Calabrian licorice roots are worldwide recognized, although it\u2019s worth noting that aerial part of this plant , usually treated as waste product, represent a surprisingly source of bioactive metabolites. Indeed distribution of metabolites depicts a peculiar biosynthetic and developmental plasticity. In this work we report the chemical profiling of surface and inner leaf content. Methods Licorice aerial parts were harvested in pre flowering and flowering time. Leaves, panicles and flowers in flowering periods were collected dried and were ultrasound extracted with EtOH \/H2O 70% three times. Vacuum dried samples were reconstituted in MeOH\/H20 1:1 for further qualitative analysis by UHPLC system. Exudates from outer licorice leaves was performed by ETOH brief immersion for 10 sec and vacuum dried , samples were analized as above. Results \/ Discussion \/ Conclusion UHPLC-ESI-HRMS analyses were performed in negative and positive ionization mode to obtain complementary information useful for characterizing the extracts of leaves, exudates, panicles and flowers in flowering periods. Metabolite assignments were made comparing UV\/Vis, HRMS and MS\/MS spectra with reference standards when available, or with chemo-taxonomic data reported in the literature and databases. The UHPLC-DAD-HRMSn analysis has allowed to identify 46 compounds belonging to two major classes of secondary metabolites: dihydrostilbenes and flavonoids. The dihydrostilbenes have the same central scaffold with different functional groups on the two aromatic rings: hydroxyl, methoxy and prenyl units. The flavonoids are glycosilated derivatives of quercetin, kaempferol, myricetin and naringenin. All extracts showed UHPLC-UV profiles qualitatively comparable except for exudate, which showed dihydrostilbenes exclusively. Licorice aerial parts are a very promising reservoir of bioactive molecules for plant itself and humans. From an ecological perspective prenilated flavonoids in the outer leaves part represent a more efficient plant protection against insects. These molecules along with inner leaf content of glycosilated flavonoids augment flavonoid bioactivity by increasing membrane affinity and improving\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.lincantore.com\/?p=1810&lang=it\" \/>\n<meta property=\"og:site_name\" content=\"l'incantore\" \/>\n<meta property=\"article:published_time\" content=\"2019-04-17T10:04:09+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2023-06-08T13:08:21+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.lincantore.com\/wp-content\/uploads\/2019\/04\/lincantore-chimali-2018-2.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"700\" \/>\n\t<meta property=\"og:image:height\" content=\"450\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"ClauNath@admin\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"\u00c9crit par\" \/>\n\t<meta name=\"twitter:data1\" content=\"ClauNath@admin\" \/>\n\t<meta name=\"twitter:label2\" content=\"Dur\u00e9e de lecture estim\u00e9e\" \/>\n\t<meta name=\"twitter:data2\" content=\"7 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/www.lincantore.com\/?p=1810&lang=it#article\",\"isPartOf\":{\"@id\":\"https:\/\/www.lincantore.com\/?p=1810&lang=it\"},\"author\":{\"name\":\"ClauNath@admin\",\"@id\":\"https:\/\/www.lincantore.com\/#\/schema\/person\/775ed2bdb1492c42484e50995cb2b1c1\"},\"headline\":\"La filiale italiana dell’Incanto, l’Incanto Italia, partecipa ad un progetto di ricerca finanziata dalla Comunit\u00e0 Europea\",\"datePublished\":\"2019-04-17T10:04:09+00:00\",\"dateModified\":\"2023-06-08T13:08:21+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/www.lincantore.com\/?p=1810&lang=it\"},\"wordCount\":1379,\"publisher\":{\"@id\":\"https:\/\/www.lincantore.com\/#organization\"},\"articleSection\":[\"Actualit\u00e9s-it\"],\"inLanguage\":\"fr-FR\"},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.lincantore.com\/?p=1810&lang=it\",\"url\":\"https:\/\/www.lincantore.com\/?p=1810&lang=it\",\"name\":\"La filiale italiana dell'Incanto, l'Incanto Italia, partecipa ad un progetto di ricerca finanziata dalla Comunit\u00e0 Europea - 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l'incantore","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.lincantore.com\/?p=1810&lang=it","og_locale":"fr_FR","og_type":"article","og_title":"La filiale italiana dell'Incanto, l'Incanto Italia, partecipa ad un progetto di ricerca finanziata dalla Comunit\u00e0 Europea - l'incantore","og_description":"La filiale italiana dell’Incanto, l’Incanto Italia, partecipa ad un progetto di ricerca finanziata dalla Comunit\u00e0 Europea per l’identificazione dei principi attivi estratti delle piante che si sviluppano nel sud dell’Italia. La ricerca ha iniziato i suoi lavori su numerosi estratti vegetali presenti in Calabria, componenti bioattivi molto interessanti dal punto di vista cosmetico, nutrizionale ma anche ambientalista. La nostra ricercatrice cos\u00ec come i ricercatori delle Universit\u00e0 di Salerno e di Reggio Calabria hanno messo in evidenza l’attivit\u00e0 antiossidante elevata della pelle della cipolla rossa di Tropea. Questa parte della cipolla, abitualmente considerata come uno scarto, \u00e8 costituita principalmente di flavono\u00efdi : requisito fondamentale per un uso pi\u00f9 sostenibile e produttivo nelle formulazioni farmaceutiche, cosmetiche e nutraceutiche. Parimenti, se la qualit\u00e0, l’aroma ed il gusto delle radici di liquirizia Calabrese sono conosciuti universalmente, le parti aeree della liquirizia abitualmente considerate come gli scarti sono state analizzate e si rivelano essere con sorpresa una sorgente di ingredienti bioattivi per la pianta lei stessa e per noi a causa della presenza di numerosi componenti che appartengono a 2 classi maggiorenni di polifenoli. Considerando le propriet\u00e0 antimicrobiche ed antinfiammatorie dei principali componenti delle parti aeree della liquirizia, \u00e8 evidente che questi \u00a0\u00bb scarti\u00a0\u00bb rappresentano una sorgente di materia prima potenziale da promovere per la salute nei settori cosmetico , nutraceutico ed alimentare. Altri studi sono in corso per valutare le loro applicazioni. I risultati di queste prime ricerche sono stati presentati ai seguenti Congressi : Congresso Mondiale annuale della Societ\u00e0 Italo-Latino- Americana di Etnomedicina, Alimentazione e Salute (SILAE) a Milazzo (Sicilia): World Ethnomedicine Summit dal 9 al 13 Settembre 2018 E Chimali – XII \u00b0 Italian Food Chemistry Congress Camerino (ITALY) 24-27 SEPTEMBRE 2018 CHIMALI – XII Italian Food Chemistry CongressCamerino, September 24-27 2018 N\u2019oublions pas le principe de l\u2019\u00cfncantore : Rechercher les ingr\u00e9dients dans la Nature pour pr\u00e9server naturellement l\u2019enchantement de la beaut\u00e9. RESUME DES ARTICLES Tropea Onion skin: from a waste product to new valuable matrix for bioactivity Rita Celano1, Teresa Docimo2, Anna Lisa Piccinelli1, Luca Campone1,4, Claudio Cannistr\u00e03, Rosa Di Sanzo4, Sonia Carabetta4 Luca Rastrelli1, Mariateresa Russo5 1Department of Pharmacy, University of Salerno; 2L\u2019incanto Sarl, Catanzaro; 3 H\u00f4pital Bichat – Claude-Bernard, Paris, Francia; 4 Food Chemistry, Authentication, Safety and Sensoromic Laboratory \u2013 Universit\u00e0 Mediterranea di Reggio Calabria Introduction The red onion from Tropea is known for its sweetly taste, which is mainly due to the flavonoids and the alk(en)yl cysteine sulphoxides (ACSOs) compounds. The unique red onion envelope usually considered a waste product, represent a valuable source of biomolecules therefore accurate chemical characterization of this matrix is a fundamental prerequisite for a more sustainable and productive use in pharmaceutical, cosmetical and nutraceutical formulations. Methods The outer layers of red skin onion bulbs were collected, dried and finely powdered. Exhaustive extraction of dried materials was performed by ultrasound-assisted extraction (UAE) with aqueous ethanol (70% v\/v). A UHPLC-HRMS\/MS method was developed and then applied to analyze the onion-skin extract. Results The chemical composition of onion-skin extract was determined by UHPLC-DAD-HRMS analysis. Metabolite assignments were made comparing UV\/Vis, HRMS and MS\/MS spectra with reference standards when available, or with chemo-taxonomic data reported in the literature and databases. The main compounds detected in the extract belonging to two major classes of secondary metabolites: anthocyanins and flavonols. The main flavonols found in this onion type were quercetin and quercetin glucosides, isorhamnetin glucosides, kaempferol glucoside, and, among anthocyanins, cyaniding glucosides. This extract has shown a high antioxidant activity. The activity antioxidant was measured by DPPH, ABTS and ORAC assays. Conclusions The obtained results have shown that the onion-skins are a promising and cheap source of bioactive compounds with high antioxidant activity. Furthermore, their use as a substitute for synthetic antioxidants, in addition to increasing food security, would contribute greatly to increase their added value. Unravelling metabolic plasticity of Glycyrrhiza glabra leaves DOP calabrese by chemical profiling Rita Celano1, Teresa Docimo2, Anna Lisa Piccinelli1, Claudio Cannistr\u00e03, Antonio Massarotto4, Salvatore Fuda5, Luca Rastrelli1, Mariateresa Russo5 1Department of Pharmacy, University of Salerno; 2 L\u2019incanto Sarl, Catanzaro; 3 H\u00f4pital Bichat – Claude-Bernard, Paris, Francia; 4 Naturemed srl, Cosenza; 5 Food Chemistry, Authentication, Safety and Sensoromic Laboratory \u2013 Universit\u00e0 Mediterranea di Reggio Calabria Introduction Quality, aroma and flavor of Calabrian licorice roots are worldwide recognized, although it\u2019s worth noting that aerial part of this plant , usually treated as waste product, represent a surprisingly source of bioactive metabolites. Indeed distribution of metabolites depicts a peculiar biosynthetic and developmental plasticity. In this work we report the chemical profiling of surface and inner leaf content. Methods Licorice aerial parts were harvested in pre flowering and flowering time. Leaves, panicles and flowers in flowering periods were collected dried and were ultrasound extracted with EtOH \/H2O 70% three times. Vacuum dried samples were reconstituted in MeOH\/H20 1:1 for further qualitative analysis by UHPLC system. Exudates from outer licorice leaves was performed by ETOH brief immersion for 10 sec and vacuum dried , samples were analized as above. Results \/ Discussion \/ Conclusion UHPLC-ESI-HRMS analyses were performed in negative and positive ionization mode to obtain complementary information useful for characterizing the extracts of leaves, exudates, panicles and flowers in flowering periods. Metabolite assignments were made comparing UV\/Vis, HRMS and MS\/MS spectra with reference standards when available, or with chemo-taxonomic data reported in the literature and databases. The UHPLC-DAD-HRMSn analysis has allowed to identify 46 compounds belonging to two major classes of secondary metabolites: dihydrostilbenes and flavonoids. The dihydrostilbenes have the same central scaffold with different functional groups on the two aromatic rings: hydroxyl, methoxy and prenyl units. The flavonoids are glycosilated derivatives of quercetin, kaempferol, myricetin and naringenin. All extracts showed UHPLC-UV profiles qualitatively comparable except for exudate, which showed dihydrostilbenes exclusively. Licorice aerial parts are a very promising reservoir of bioactive molecules for plant itself and humans. From an ecological perspective prenilated flavonoids in the outer leaves part represent a more efficient plant protection against insects. These molecules along with inner leaf content of glycosilated flavonoids augment flavonoid bioactivity by increasing membrane affinity and improving","og_url":"https:\/\/www.lincantore.com\/?p=1810&lang=it","og_site_name":"l'incantore","article_published_time":"2019-04-17T10:04:09+00:00","article_modified_time":"2023-06-08T13:08:21+00:00","og_image":[{"width":700,"height":450,"url":"https:\/\/www.lincantore.com\/wp-content\/uploads\/2019\/04\/lincantore-chimali-2018-2.jpg","type":"image\/jpeg"}],"author":"ClauNath@admin","twitter_card":"summary_large_image","twitter_misc":{"\u00c9crit par":"ClauNath@admin","Dur\u00e9e de lecture estim\u00e9e":"7 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/www.lincantore.com\/?p=1810&lang=it#article","isPartOf":{"@id":"https:\/\/www.lincantore.com\/?p=1810&lang=it"},"author":{"name":"ClauNath@admin","@id":"https:\/\/www.lincantore.com\/#\/schema\/person\/775ed2bdb1492c42484e50995cb2b1c1"},"headline":"La filiale italiana dell’Incanto, l’Incanto Italia, partecipa ad un progetto di ricerca finanziata dalla Comunit\u00e0 Europea","datePublished":"2019-04-17T10:04:09+00:00","dateModified":"2023-06-08T13:08:21+00:00","mainEntityOfPage":{"@id":"https:\/\/www.lincantore.com\/?p=1810&lang=it"},"wordCount":1379,"publisher":{"@id":"https:\/\/www.lincantore.com\/#organization"},"articleSection":["Actualit\u00e9s-it"],"inLanguage":"fr-FR"},{"@type":"WebPage","@id":"https:\/\/www.lincantore.com\/?p=1810&lang=it","url":"https:\/\/www.lincantore.com\/?p=1810&lang=it","name":"La filiale italiana dell'Incanto, l'Incanto Italia, partecipa ad un progetto di ricerca finanziata dalla Comunit\u00e0 Europea - 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La ricerca ha iniziato i suoi lavori su numerosi estratti vegetali presenti in Calabria, componenti bioattivi molto interessanti dal punto di vista cosmetico, nutrizionale ma anche ambientalista. La nostra ricercatrice cos\u00ec come i ricercatori delle Universit\u00e0 di Salerno e di Reggio Calabria hanno messo in evidenza l’attivit\u00e0 antiossidante elevata della pelle della cipolla rossa di Tropea. Questa parte della cipolla, abitualmente considerata come uno scarto, \u00e8 costituita principalmente di flavono\u00efdi : requisito fondamentale per un uso pi\u00f9 sostenibile e produttivo nelle formulazioni farmaceutiche, cosmetiche e nutraceutiche.<\/p>\n<p><!-- \/wp:paragraph --><\/p>\n<p><!-- wp:paragraph --><\/p>\n<p>Parimenti, se la qualit\u00e0, l’aroma ed il gusto delle radici di liquirizia Calabrese sono conosciuti universalmente, le parti aeree della liquirizia abitualmente considerate come gli scarti sono state analizzate e si rivelano essere con sorpresa una sorgente di ingredienti bioattivi per la pianta lei stessa e per noi a causa della presenza di numerosi componenti che appartengono a 2 classi maggiorenni di polifenoli.<\/p>\n<p><!-- \/wp:paragraph --><\/p>\n<p><!-- wp:paragraph --><\/p>\n<p>Considerando le propriet\u00e0 antimicrobiche ed antinfiammatorie dei principali componenti delle parti aeree della liquirizia, \u00e8 evidente che questi \u00a0\u00bb scarti\u00a0\u00bb rappresentano una sorgente di materia prima potenziale da promovere per la salute nei settori cosmetico , nutraceutico ed alimentare. Altri studi sono in corso per valutare le loro applicazioni.<\/p>\n<p><!-- \/wp:paragraph --><\/p>\n<p><!-- wp:paragraph --><\/p>\n<p>I risultati di queste prime ricerche sono stati presentati ai seguenti Congressi :<\/p>\n<p><!-- \/wp:paragraph --><\/p>\n<p><!-- wp:paragraph --><\/p>\n<p><strong>Congresso Mondiale annuale della Societ\u00e0 Italo-Latino- Americana di Etnomedicina, Alimentazione e Salute (SILAE) a Milazzo (Sicilia): World Ethnomedicine Summit dal 9 al 13 Settembre 2018<\/strong><\/p>\n<p><!-- \/wp:paragraph --><\/p>\n<p><!-- wp:image {\"align\":\"center\"} --><\/p>\n<figure class=\"wp-block-image aligncenter\"><img src=\"https:\/\/www.lincantore.com\/images\/SILAE-2018.jpg\" alt=\"https:\/\/www.velp.com\/euronet\/contenuti\/image\/events\/SILAE18_600(1).jpg\" \/><\/figure>\n<p><!-- \/wp:image --><\/p>\n<p><!-- wp:paragraph --><\/p>\n<p>E<\/p>\n<p><!-- \/wp:paragraph --><\/p>\n<p><!-- wp:paragraph --><\/p>\n<p><strong><u>Chimali – XII \u00b0 Italian Food Chemistry Congress<\/u><\/strong><\/p>\n<p><strong><u>Camerino (ITALY) 24-27 SEPTEMBRE 2018<\/u><\/strong><\/p>\n<p><!-- \/wp:paragraph --><\/p>\n<p><!-- wp:paragraph {\"align\":\"center\"} --><\/p>\n<p class=\"has-text-align-center\"><img src=\"https:\/\/www.lincantore.com\/images\/Chimali-2018.png\" alt=\"Home\" width=\"50%\"><\/p>\n<p><a href=\"http:\/\/chimali2018.unicam.it\/\"><strong>CHIMALI – XII Italian Food Chemistry Congress<\/strong><\/a><br \/><strong>Camerino, September 24-27 2018<\/strong><\/p>\n<p><!-- \/wp:paragraph --><\/p>\n<p><!-- wp:paragraph --><\/p>\n<p><strong>N\u2019oublions pas le principe de l\u2019\u00cfncantore :<\/strong><\/p>\n<p><strong> Rechercher les ingr\u00e9dients dans la Nature pour pr\u00e9server naturellement l\u2019enchantement de la beaut\u00e9.<\/strong><\/p>\n<p><!-- \/wp:paragraph --><\/p>\n<p><!-- wp:paragraph --><\/p>\n<p><strong><u>RESUME DES ARTICLES<\/u><\/strong><\/p>\n<p><!-- \/wp:paragraph --><\/p>\n<p><!-- wp:paragraph --><\/p>\n<p><strong><u>Tropea Onion skin: from a waste product to new valuable matrix for bioactivity<\/u><\/strong><\/p>\n<p><!-- \/wp:paragraph --><\/p>\n<p><!-- wp:paragraph --><\/p>\n<p>Rita Celano1, Teresa Docimo2, Anna Lisa Piccinelli1, Luca Campone1,4, Claudio Cannistr\u00e03, Rosa Di Sanzo4, Sonia Carabetta4 Luca Rastrelli1, Mariateresa Russo5<\/p>\n<p>1Department of Pharmacy, University of Salerno; 2L\u2019incanto Sarl, Catanzaro; 3 H\u00f4pital Bichat – Claude-Bernard, Paris, Francia; 4 Food Chemistry, Authentication, Safety and Sensoromic Laboratory \u2013 Universit\u00e0 Mediterranea di Reggio Calabria<\/p>\n<p><strong>Introduction<\/strong><\/p>\n<p>The red onion from Tropea is known for its sweetly taste, which is mainly due to the flavonoids and the alk(en)yl cysteine sulphoxides (ACSOs) compounds. The unique red onion envelope usually considered a waste product, represent a valuable source of biomolecules therefore accurate chemical characterization of this matrix is a fundamental prerequisite for a more sustainable and productive use in pharmaceutical, cosmetical and nutraceutical formulations.<\/p>\n<p><strong>Methods<\/strong><\/p>\n<p>The outer layers of red skin onion bulbs were collected, dried and finely powdered. Exhaustive extraction of dried materials was performed by ultrasound-assisted extraction (UAE) with aqueous ethanol (70% v\/v). A UHPLC-HRMS\/MS method was developed and then applied to analyze the onion-skin extract.<\/p>\n<p><strong>Results<\/strong><\/p>\n<p>The chemical composition of onion-skin extract was determined by UHPLC-DAD-HRMS analysis. Metabolite assignments were made comparing UV\/Vis, HRMS and MS\/MS spectra with reference standards when available, or with chemo-taxonomic data reported in the literature and databases. The main compounds detected in the extract belonging to two major classes of secondary metabolites: anthocyanins and flavonols. The main flavonols found in this onion type were quercetin and quercetin glucosides, isorhamnetin glucosides, kaempferol glucoside, and, among anthocyanins, cyaniding glucosides. This extract has shown a high antioxidant activity. The activity antioxidant was measured by DPPH, ABTS and ORAC assays.<\/p>\n<p><strong>Conclusions<\/strong><\/p>\n<p>The obtained results have shown that the onion-skins are a promising and cheap source of bioactive compounds with high antioxidant activity. Furthermore, their use as a substitute for synthetic antioxidants, in addition to increasing food security, would contribute greatly to increase their added value.<\/p>\n<p><!-- \/wp:paragraph --><\/p>\n<p><!-- wp:separator --><\/p>\n<hr class=\"wp-block-separator has-alpha-channel-opacity\" \/>\n<!-- \/wp:separator --><\/p>\n<p><!-- wp:paragraph --><\/p>\n<p><strong><u>Unravelling metabolic plasticity of Glycyrrhiza glabra leaves DOP calabrese by chemical profiling<\/u><\/strong><\/p>\n<p><!-- \/wp:paragraph --><\/p>\n<p><!-- wp:paragraph --><\/p>\n<p>Rita Celano1, Teresa Docimo2, Anna Lisa Piccinelli1, Claudio Cannistr\u00e03, Antonio Massarotto4, Salvatore Fuda5, Luca Rastrelli1, Mariateresa Russo5<\/p>\n<p>1Department of Pharmacy, University of Salerno;<\/p>\n<p>2 L\u2019incanto Sarl, Catanzaro; 3 H\u00f4pital Bichat – Claude-Bernard, Paris, Francia; 4 Naturemed srl, Cosenza; 5 Food Chemistry, Authentication, Safety and Sensoromic Laboratory \u2013 Universit\u00e0 Mediterranea di Reggio Calabria<\/p>\n<p><strong>Introduction<\/strong><\/p>\n<p>Quality, aroma and flavor of Calabrian licorice roots are worldwide recognized, although it\u2019s worth noting that aerial part of this plant , usually treated as waste product, represent a surprisingly source of bioactive metabolites. Indeed distribution of metabolites depicts a peculiar biosynthetic and developmental plasticity. In this work we report the chemical profiling of surface and inner leaf content.<\/p>\n<p><strong>Methods<\/strong><\/p>\n<p>Licorice aerial parts were harvested in pre flowering and flowering time. Leaves, panicles and flowers in flowering periods were collected dried and were ultrasound extracted with EtOH \/H2O 70% three times. Vacuum dried samples were reconstituted in MeOH\/H20 1:1 for further qualitative analysis by UHPLC system. Exudates from outer licorice leaves was performed by ETOH brief immersion for 10 sec and vacuum dried , samples were analized as above.<\/p>\n<p><strong>Results \/ Discussion \/ Conclusion<\/strong><\/p>\n<p>UHPLC-ESI-HRMS analyses were performed in negative and positive ionization mode to obtain complementary information useful for characterizing the extracts of leaves, exudates, panicles and flowers in flowering periods. Metabolite assignments were made comparing UV\/Vis, HRMS and MS\/MS spectra with reference standards when available, or with chemo-taxonomic data reported in the literature and databases. The UHPLC-DAD-HRMSn analysis has allowed to identify 46 compounds belonging to two major classes of secondary metabolites: dihydrostilbenes and flavonoids. The dihydrostilbenes have the same central scaffold with different functional groups on the two aromatic rings: hydroxyl, methoxy and prenyl units. The flavonoids are glycosilated derivatives of quercetin, kaempferol, myricetin and naringenin. All extracts showed UHPLC-UV profiles qualitatively comparable except for exudate, which showed dihydrostilbenes exclusively.<\/p>\n<p>Licorice aerial parts are a very promising reservoir of bioactive molecules for plant itself and humans. From an ecological perspective prenilated flavonoids in the outer leaves part represent a more efficient plant protection against insects. These molecules along with inner leaf content of glycosilated flavonoids augment flavonoid bioactivity by increasing membrane affinity and improving the interaction with target proteins.<\/p>\n<p><!-- \/wp:paragraph --><\/p>\n<p><!-- wp:separator --><\/p>\n<hr class=\"wp-block-separator has-alpha-channel-opacity\" \/>\n<!-- \/wp:separator --><\/p>\n<p><!-- wp:paragraph --><\/p>\n<p><strong><u>Chemical profiling of Glycyrrhiza glabra aerial parts, a waste product of PDO<\/u><\/strong><\/p>\n<p><strong><u>\u2018\u2018Licorice of Calabria\u2019\u2019<\/u><\/strong><\/p>\n<p>Rita Celano1, Teresa Docimo2, Anna Lisa Piccinelli1, Claudio Cannistr\u00e03, Antonio Massarotto4, Salvatore Fuda5, Luca Rastrelli1, Mariateresa Russo5<\/p>\n<p>1Department of Pharmacy, University of Salerno; 2 L\u2019incanto Sarl, Catanzaro; 3 H\u00f4pital Bichat Claude Bernard, Paris, Francia; 4 Naturemed srl, Cosenza; 5 Food Chemistry, Authentication, Safety andSensoromic Laboratory \u2013 Universit\u00e0 Mediterranea di Reggio Calabria<\/p>\n<p><strong>Introduction<\/strong><\/p>\n<p>Glycyrrhiza glabra aerial parts are a huge amount of discarded material produced during the cultivation and harvest of PDO \u2018\u2018Licorice of Calabria\u2019\u2019 roots. This waste product represents a potential source of functional and health-promoting ingredients. The chemical profiles of exudates, leaves, panicles and flowers were determined by UHPLC-HRMS to estimate their nutraceutical potential.<\/p>\n<p><strong>Methods<\/strong><\/p>\n<p>G. glabra aerial parts were harvested in pre-flowering and flowering time from three PDO cultivations in Calabria. Exhaustive extraction of dried materials was performed by ultrasound-assisted extraction (UAE) with aqueous ethanol (70% v\/v). Exudates were recovered by immersion of leaves in ethanol (10 sec). A UHPLC-HRMS\/MS method was developed and then applied to analyze the aerial part extracts.<\/p>\n<p><strong>Results<\/strong><\/p>\n<p>Negative and positive UHPLC-HRMS analyses, combined with HRMS\/MS spectra, chemo-taxonomic data and databases, allowed the full characterization of the metabolites of Calabrian G. glabra leaf exudate, leaf, panicle and flowers. 46 phenolic compounds were identified in aerial parts belonging to two major secondary metabolite classes: dihydrostilbenes and flavonoids. Dihydrostilbenes have the same central scaffold with different functional groups on the aromatic rings (methoxy and prenyl units). Identified flavonoids are flavanones (naringenin an pinocembrin) and their prenylated derivatives, C- and O-glycosilated flavones and flavonols. Dihydrostilbenes, pinocembrin and prenylated flavanones are the most abundant bioactive compounds of aerial parts and they represent the distinctive metabolites of leaf exudates. Instead, glycosylated flavonoids are present in inner leaves and flowers.<\/p>\n<p><strong>Conclusions<\/strong><\/p>\n<p>UHPLC-HRMS profiling prove that G. glabra aerial parts are a suitable and rich source of bioactive compounds. Their main compounds have well known antimicrobic and antinflammatory properties and it is evident that these wastes represent a potential raw material to recover health-promoting ingredients for food, cosmetic and nutraceutical sectors. Further studies are in progress to evaluate their potential applications.<\/p>\n<p><!-- \/wp:paragraph --><\/p>\n","comment_info":"0","pll_sync_post":[],"_links":{"self":[{"href":"https:\/\/www.lincantore.com\/index.php?rest_route=\/wp\/v2\/posts\/1810","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.lincantore.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.lincantore.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.lincantore.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.lincantore.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1810"}],"version-history":[{"count":2,"href":"https:\/\/www.lincantore.com\/index.php?rest_route=\/wp\/v2\/posts\/1810\/revisions"}],"predecessor-version":[{"id":1820,"href":"https:\/\/www.lincantore.com\/index.php?rest_route=\/wp\/v2\/posts\/1810\/revisions\/1820"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.lincantore.com\/index.php?rest_route=\/wp\/v2\/media\/2466"}],"wp:attachment":[{"href":"https:\/\/www.lincantore.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1810"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.lincantore.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1810"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.lincantore.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1810"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
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